Reverse Transcription by Escherichia coli DNA Polymerase I
نویسندگان
چکیده
منابع مشابه
On the processive mechanism of Escherichia coli DNA polymerase I.
A procedure has been developed to assess whether polymerization of nucleotides by DNA polymerases is processive, that is, whether a succession of polymerization steps occurs without release of the enzyme from the template. The method involves measurement of the ratio of deoxyguanylate to deocycytidylate incorporated in the course of replicating a segment of the right hand cohesive end of phage ...
متن کاملDetection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
متن کاملShort-patch reverse transcription in Escherichia coli.
Chimeras of RNA and DNA have distinctive physical and biological properties. Chimeric oligonucleotides that contained one, two or three ribonucleotides whose phosphodiester backbone was covalently continuous with DNA were synthesized. Site-directed mutagenesis was used to assess genetic information transfer from the ribonucleotide positions. Transfer was scored by the formation or reversion of ...
متن کاملTranscription pausing by Escherichia coli RNA polymerase is modulated by downstream DNA sequences.
Escherichia coli RNA polymerase pauses immediately after transcription of certain sequences that can form stable secondary structures in the nascent RNA transcript; pausing appears to be essential for several types of bacterial transcription attenuation mechanisms. Because base changes that weaken the RNA secondary structures reduce the half-life of pausing by RNA polymerase, nascent transcript...
متن کاملRecognition of B and Z forms of DNA by Escherichia coli DNA polymerase I.
Since the substrate binding domain of the large proteolytic fragment of Escherichia coli DNA polymerase I has been shown to interact with the B forms of DNA, we have studied the ability of this enzyme to recognize structures other than the B form. The polymerase activity has been used to evaluate the degree of recognition of the B and Z forms of DNA. The Z form was found to promote less activit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1973
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.70.12.3834